RNA Workflow Products

End-to-end support of your RNA workflow

NucleoProtect RNA

Collect samples now. Extract RNA later. With Macherey-Nagel’s RNA stabilization reagent, you can skip the flash freeze, easily store and transport tissue samples and cells, and extract RNA on your schedule.

RNA is extremely sensitive to degradation, but it’s not always practical to extract RNA at the time when tissue is sampled. Traditionally, researchers have turned to flash freezing the tissue to preserve RNA for later extraction. NucleoProtect is a more innovative alternative that doesn’t tie you to a liquid nitrogen tank. 

The NucleoProtect reagent from Macherey-Nagel permeates through up to 5mm of tissue, aggregated nucleases, and fixes RNA without any toxic side effects. RNA can be recovered from tissue submerged in NucleoProtect months or years later.

  • RNA preserved through 10 freeze-thaw cycles
  • Stable for a week in the fridge or a month in the freezer
  • Stable long term at -20 °C
  • Compatible with most RNA isolation methods

NucleoMag Pathogen Kits

Efficient purification of viral RNA/DNA and microbial DNA with magnetic beads.  The NucleoMag Pathogen kit allows rapid manual or automated small-scale purification of viral RNA /DNA using magnetic bead technology. Designed for processing and purification of common clinical sample types, NucleoMag kits come equipped with ready-to-use carrier RNA, proteinase K, and all buffers. The NucleoMag technology can easily be adapted to common liquid handling instruments. It can be used either with static pin separators or magnetic separators integrated into robotic workstations. Start with faster and more precise automated sample purification today.

  • Magnetic bead-based nucleic acid isolation
  • 96 samples per kit
  • Compatible with automated workflows
  • <2 hour prep time
  • Simple 1-tube procedure

NucleoSpin RNA

Recommended isolation and purification of total RNA. NucleoSpin RNA is recommended for isolation and purification of total RNA from cultured cells, tissue, bacteria, yeast, cell-free biological fluids, and reaction mixtures without the use of organic compounds (e.g., Trizol). This kit allows purification of up to 70 µg of highly pure, DNA-free total RNA. Total RNA prepared with NucleoSpin RNA is suitable for applications such as real-time reverse transcription PCR (qRT-PCR), Northern blotting, primer extension, array technology, and RNase protection assays.

  • High integrity isolated RNA
  • Even biological samples which are sometimes difficult to process will yield high quality RNA.
  • The isolated RNA is ready to use for applications like reverse transcriptase-PCR (RT-PCR), primer extension, or RNase protection assays.
  • Significantly reduced DNA contamination

Lexogen RNA
Library Prep Kits

CORALL Total RNA-Seq Library Prep Kit

CORALL Total RNA-Seq Library Prep Kit

Enable streamlined generation of Illumina-compatible libraries within 4.5 hours. CORALL is Lexogen’s new stranded library prep kit. Achieve excellent whole transcriptome coverage with this kit, which uses a fragmentation-free protocol to deliver complete transcript representation, including start and end sites. This is a great option for generating comprehensive Illumina-compatible libraries.

  • Complete coverage of transcripts from start to end
  • Ready-to-sequence libraries within 4.5 hours
  • Unique Molecular Identifiers (UMIs) seamlessly included
  • Excellent protocol-inherent strandedness (>99 %)
  • 1 ng to 1 μg of total RNA input
  • Easy all-in-one protocol

QuantSeq 3’ mRNA-Seq Library Prep Kit REV for Illumina

QuantSeq 3' mRNA-Seq Library Prep Kit REV for Illumina

Generate Illumina-compatible libraries of sequences at the 3′ end of the polyadenylated RNA. Pinpoint the 3′ end in Read 1 to study the 3′ UTR and alternative polyadenylation. The QuantSeq REV kit requires an input amount of total RNA as low as 10 ng to reproducibly generate libraries from low quality RNA, including FFPE samples. The simple workflow can generate ready-to-sequence, Illumina-compatible NGS libraries in as little as 4.5 hours, with less than 2 hours hands-on. Start today.

  • Complete coverage of transcripts from start to end
  • Ready-to-sequence libraries within 4.5 hours
  • Unique Molecular Identifiers (UMIs) seamlessly included
  • Excellent protocol-inherent strandedness (>99 %)
  • 1 ng to 1 μg of total RNA input
  • Easy all-in-one protocol