Reverse transcription and real-time PCR have always been close partners. With PCR Biosystem’s SyGreen 1-Step Kits, RT and qPCR are coupled into a simple 1 tube, 1 step protocol.
PCR Biosystems offers two unique qPCR Sygreen 1-Step kits to meet your project needs combining an antibody- mediated hot start with a highly active MMLV reverse transcriptase for improved reaction sensitivity and specificity that remain thermostable at temperatures up to 45-55°C.
qPCR 1-Step Kits are the simple, no-hassle solution to an efficient and seamless qPCR workflow. Kits are equipped with a modified SyGreen intercalating dye that doesn’t inhibit PCR or reverse transcription while buffers are optimized in high-throughput screens to promote more efficient cycling.
And the best part? Little to no optimization required.
For low copy number targets
qPCRBIO SyGreen 1-Step Detect kits are engineered for high sensitivity (10pg -100ng total RNA or >0.01pg mRNA).